pfkfb3 inhibitor 3po (MedChemExpress)
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pfkfb3 inhibitor 3po
Pfkfb3 Inhibitor 3po, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfkfb3+inhibitor+3po/3PO/pmc13083170-63-1-5
Average 95 stars, based on 38 article reviews
Pfkfb3 Inhibitor 3po, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfkfb3+inhibitor+3po/3PO/pmc13083170-63-1-5
Average 95 stars, based on 38 article reviews
pfkfb3 inhibitor 3po - by Bioz Stars,
2026-10
95/100 stars
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1) Product Images from "Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments"
Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments
Journal: Frontiers in Pharmacology
doi: 10.3389/fphar.2026.1790273
Figure Legend Snippet: Network pharmacology analysis of the PFKFB3 inhibitor 3PO’s potential anti-asthma targets. (A) The chemical structure of 3PO. (B) Venn diagram illustrating 43 overlapping targets between 3PO-predicted targets and asthma-related genes. (C) PPI network constructed from the 43 intersecting targets. (D) Top 9 hub genes identified and visualized in Cytoscape based on degree centrality: node color intensity (red) corresponds to higher connectivity. (E) GO enrichment analysis results, including BP, CC, and MF. (F) KEGG enrichment analysis results. Protein-protein interaction = PPI, GO = Gene ontology, BP = Biological process, CC = Cellular component, MF = Molecular function, KEGG = Kyoto encyclopedia of genes and genomes.
Techniques Used: Construct
Figure Legend Snippet: Molecular docking results of 3PO with the top eight core targets. (A) Molecular docking of 3PO with IL-1β. (B) Molecular docking of 3PO with IL-6. (C) Molecular docking of 3PO with TNF-α. (D) Molecular docking of 3PO with NF-κB p65. (E) Molecular docking of 3PO with Bcl-2. (F) Molecular docking of 3PO with Caspase3. (G) Molecular docking of 3PO with Tau. (H) Molecular docking of 3PO with MAO-B. IL-1β = Interleukin-1β, IL-6 = Interleukin-6, TNF-α = Tumor necrosis factor-α, NF-κB = Nuclear factor kappa B.
Techniques Used:
Figure Legend Snippet: The molecular dynamics simulations profiles of the IL-1β-3PO complex. (A) RMSD values (Complex: IL-1β-3PO, Protein: IL-1β, Ligand: 3PO). (B) RMSF analysis. (C) Number of hydrogen bonds formed between IL-1β and 3PO during the simulation. (D) Rg analysis. (E) SASA analysis. (F) Gibbs free energy landscape (2D). (G) Gibbs free energy landscape (3D). (H) The lowest energy 3D conformation. (I) 2D patterns of bonds at the most stable condition of the complex. (J) Time evolution of the MM/GBSA binding free energy. (K) Decomposition of the MM/GBSA binding free energy. (L) Per-residue energy decomposition. RMSD = Root mean square deviation, RMSF = Root mean square fluctuation, Rg = Radius of gyration, SASA = Solvent accessible surface area.
Techniques Used: Binding Assay, Residue, Solvent
Figure Legend Snippet: The molecular dynamics simulations profiles of the IL-6-3PO complex. (A) RMSD values (Complex: IL-6-3PO, Protein: IL-6, Ligand: 3PO). (B) RMSF analysis. (C) Number of hydrogen bonds formed between IL-6 and 3PO during the simulation. (D) Rg analysis. (E) SASA analysis. (F) Gibbs free energy landscape (2D). (G) Gibbs free energy landscape (3D). (H) The lowest energy 3D conformation. (I) 2D patterns of bonds at the most stable condition of the complex. (J) Time evolution of the MM/GBSA binding free energy. (K) Decomposition of the MM/GBSA binding free energy. (L) Per-residue energy decomposition.
Techniques Used: Binding Assay, Residue
Figure Legend Snippet: The molecular dynamics simulations profiles of the TNF-α-3PO complex. (A) RMSD values (Complex: TNF-α-3PO, Protein: TNF-α, Ligand: 3PO). (B) RMSF analysis. (C) Number of hydrogen bonds formed between TNF-α and 3PO during the simulation. (D) Rg analysis. (E) SASA analysis. (F) Gibbs free energy landscape (2D). (G) Gibbs free energy landscape (3D). (H) The lowest energy 3D conformation. (I) 2D patterns of bonds at the most stable condition of the complex. (J) Time evolution of the MM/GBSA binding free energy. (K) Decomposition of the MM/GBSA binding free energy. (L) Per-residue energy decomposition.
Techniques Used: Binding Assay, Residue
Figure Legend Snippet: The molecular dynamics simulations profiles of NF-κB p65-3PO complex. (A) RMSD values (Complex: NF-κB p65-3PO, Protein: NF-κB p65 Ligand: 3PO). (B) RMSF analysis. (C) Number of hydrogen bonds formed between NF-κB p65 and 3PO during the simulation. (D) Rg analysis. (E) SASA analysis. (F) Gibbs free energy landscape (2D). (G) Gibbs free energy landscape (3D). (H) The lowest energy 3D conformation. (I) 2D patterns of bonds at the most stable condition of the complex. (J) Time evolution of the MM/GBSA binding free energy. (K) Decomposition of the MM/GBSA binding free energy. (L) Per-residue energy decomposition.
Techniques Used: Binding Assay, Residue
Figure Legend Snippet: PFKFB3 inhibitor 3PO attenuated airway inflammation in OVA-induced asthmatic mice. (A) A schematic diagram of the design of animal experiments. Mice were sensitized and challenged with OVA, followed by treatment with 3PO. (B) Survival rate of mice during the experiment (no mortality observed). (C) Body weight changes of mice throughout the protocol. (D) PFKFB3 expression in lung sections was detected by immunohistochemistry. Red arrows indicate PFKFB3-positive cells. Scale bar = 50 μm. (E) Quantitative analysis of PFKFB3-positive cells in lung tissues. (F–M) Levels of cytokines in BALF, including (F) IL-4, (G) IL-5, (H) IL-13, (I) IL-17A, (J) IL-1β, (K) TNF-α, (L) IL-6, and (M) IL-10. Data are presented as the mean ± SD (n = 5 per group). #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. the control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the OVA group. BALF = Bronchoalveolar lavage fluid. IL-4 = Interleukin-4, IL-5 = Interleukin-5, IL-13 = Interleukin-13, IL-17A = Interleukin-17A, IL-10 = Interleukin-10.
Techniques Used: Expressing, Immunohistochemistry, Control
Figure Legend Snippet: PFKFB3 inhibitor 3PO attenuated airway inflammation in OVA-induced asthmatic mice. (A) Lactate levels, (B) IgE levels, and (C) CCL11 levels in BALF. (D) Total cell counts in BALF. (E) Representative images of H&E staining of lung tissue sections. Black arrows indicate eosinophils. Magnification: ×200 (upper panel) and 400× (lower panel). Scale bar = 50 μm. (F) Quantitative analysis of PAS-positive area in lung tissues. (G) Airway mucus secretion and goblet cell hyperplasia were detected using PAS staining. Scale bar = 50 μm. (H) Quantitative inflammatory cell infiltration scores. (I) Representative immunofluorescence staining for CCR3 (green) in bronchial regions; nuclei were stained with DAPI (blue). Magnification: ×200 (left panel) and 400× (right panel). Scale bar = 50 μm. Data are presented as the mean ± SD (n = 5 per group). ###P < 0.001, ####P < 0.0001 vs. the control group. **p < 0.01, ****p < 0.0001 vs. the OVA group. IgE = Immunoglobulin E, CCR3 = Chemokine (C-C motif) receptor 3, H&E = Hematoxylin and eosin, PAS = Periodic acid-Schiff, CCL11 = Chemokine (C-C motif) ligand 11.
Techniques Used: Staining, Immunofluorescence, Control
Figure Legend Snippet: PFKFB3 inhibitor 3PO inhibited the NF-κB signaling pathway activation in the OVA-induced asthma mouse models. (A) Western blot analysis of p-NF-κB P65, NF-κB P65, IKB, and β-actin protein expression. (B–D) Quantitative analysis of (B) p-NF-κB P65, (C) total NF-κB P65, and (D) IKB protein expression, normalized to β-actin. (E) Schematic diagram illustrating the effect of 3PO on OVA-induced airway inflammation in asthma. Data are presented as the mean ± SD. #P < 0.05, ##P < 0.01 vs. the control group. *p < 0.05 vs. the OVA group. ns = no significance. IKB = Inhibitor of nuclear factor kappa B.
Techniques Used: Activation Assay, Western Blot, Expressing, Control
Related Articles
Construct:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Binding Assay:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Residue:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Solvent:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Expressing:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Immunohistochemistry:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Control:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Staining:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Immunofluorescence:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Activation Assay:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The Western Blot:Article Title: Anti-inflammatory effects of 3PO in asthmatic airway inflammation: an integrated study using network pharmacology, molecular modelling, and in vivo experiments Article Snippet: The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).The primary antibodies were as follows: anti-chemokine (C-C motif) receptor 3 (CCR3) (22351-1-AP, Proteintech), anti-p-NF-κB p65 (TP56372, Abmart), anti-NF-κB p65 ( T55034 , Abmart), and anti-IKB-α ( T55026 , Abmart).. The |
